Journal: OncoTargets and therapy

Article Title: Long Noncoding RNA HEIH Promotes Proliferation, Migration and Invasion of Retinoblastoma Cells Through miR-194-5p/WEE1 Axis

doi: 10.2147/OTT.S268942

Figure Lengend Snippet: The Relationship Between HEIH Expression and Clinicopathologic Features of Retinoblastoma Patients

Article Snippet: Human normal retinal epithelial cell line ARPE-19 and retinoblastoma cell lines (Y79 and SO-Rb50) were purchased from ATCC and maintained in RPMI-1640 medium supplemented with 10% FBS at 37°C in 5% CO2. si-RNA targeting HEIH, and corresponding negative control si-NC, miR-194-5p mimics, inhibitor and negative control, WEE1 overexpressing vector pcDNA3.1- WEE1 and negative control pcDNA3.1 were supplied by RiboBio (Guangzhou, China).

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Long Noncoding RNA HEIH Promotes Proliferation, Migration and Invasion of Retinoblastoma Cells Through miR-194-5p/WEE1 Axis

doi: 10.2147/OTT.S268942

Figure Lengend Snippet: HEIH up-regulation was associated with TNM stage, optic nerve invasion and choroidal invasion in retinoblastoma. ( A ) Relative expression level of HEIH in retinoblastoma cell lines (Y79 and SO-Rb50) and normal retinal epithelial cell line ARPE-19. ( B ) Relative expression level of HEIH in 35 retinoblastoma tissues and 7 normal retinal tissues. ( C ) Relative expression level of HEIH in retinoblastoma patients with different tumor ICRB stages. ( D ) Relative expression level of HEIH in retinoblastoma patients with or without optic nerve invasion. ( E ) Relative expression level of HEIH in retinoblastoma patients with or without choroidal invasion. ( F ) The 35 retinoblastoma patients were divided into two groups (high HEIH and low HEIH) according to the median value of HEIH expression. Then we analyzed the correlation of up-regulation of HEIH with overall survival of the patients used Kaplan-Meier method and Log rank test. *P<0.05; **P<0.01.

Article Snippet: Human normal retinal epithelial cell line ARPE-19 and retinoblastoma cell lines (Y79 and SO-Rb50) were purchased from ATCC and maintained in RPMI-1640 medium supplemented with 10% FBS at 37°C in 5% CO2. si-RNA targeting HEIH, and corresponding negative control si-NC, miR-194-5p mimics, inhibitor and negative control, WEE1 overexpressing vector pcDNA3.1- WEE1 and negative control pcDNA3.1 were supplied by RiboBio (Guangzhou, China).

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Long Noncoding RNA HEIH Promotes Proliferation, Migration and Invasion of Retinoblastoma Cells Through miR-194-5p/WEE1 Axis

doi: 10.2147/OTT.S268942

Figure Lengend Snippet: HEIH knockdown inhibited retinoblastoma cell proliferation, migration and invasion. ( A ) Relative expression level of HEIH in Y79 and SO-Rb50 cells transfected with si-HEIH or siRNA control. ( B ) Cell viability was detected using the trypan blue exclusion method in Y79 and SO-Rb50 cells transfected with si-HEIH or siRNA control. ( C and D ) Cell growth capacity was evaluated using the colony formation assay. ( E and F ) Effects of HEIH knockdown on cell migration were determined using wound-healing assays. ( G and H ) Effects of HEIH knockdown on cell invasion were determined using transwell invasion assays. **P<0.01.

Article Snippet: Human normal retinal epithelial cell line ARPE-19 and retinoblastoma cell lines (Y79 and SO-Rb50) were purchased from ATCC and maintained in RPMI-1640 medium supplemented with 10% FBS at 37°C in 5% CO2. si-RNA targeting HEIH, and corresponding negative control si-NC, miR-194-5p mimics, inhibitor and negative control, WEE1 overexpressing vector pcDNA3.1- WEE1 and negative control pcDNA3.1 were supplied by RiboBio (Guangzhou, China).

Techniques: Knockdown, Migration, Expressing, Transfection, Control, Colony Assay

Journal: OncoTargets and therapy

Article Title: Long Noncoding RNA HEIH Promotes Proliferation, Migration and Invasion of Retinoblastoma Cells Through miR-194-5p/WEE1 Axis

doi: 10.2147/OTT.S268942

Figure Lengend Snippet: HEIH negatively regulated miR-194-5p expression in retinoblastoma. ( A ) Bioinformatics analysis revealed that HEIH contained putative binding site for miR-194-5p using starBase v3.0. ( B and C ) Relative luciferase activity in Y79 ( B ) and SO-Rb50 ( C ) cells co-transfected with wt-HEIH or mut-HEIH with miR-194-5p mimics or miRNA negative control. ( D ) Relative expression level of miR-194-5p in Y79 and SO-Rb50 cells transfected with si-HEIH or siRNA control. ( E ) Relative expression level of HEIH in Y79 and SO-Rb50 cells transfected with miR-194-5p mimics or miRNA negative control. ( F ) Relative expression level of miR-194-5p in 35 retinoblastoma tissues and 7 normal retinal tissues. ( G ) Relative expression level of miR-194-5p in retinoblastoma cell lines (Y79 and SO-Rb50) and normal retinal epithelial cell line ARPE-19. ( H ) Pearson correlation analysis between HEIH and miR-194-5p level in retinoblastoma tissues. **P<0.01.

Article Snippet: Human normal retinal epithelial cell line ARPE-19 and retinoblastoma cell lines (Y79 and SO-Rb50) were purchased from ATCC and maintained in RPMI-1640 medium supplemented with 10% FBS at 37°C in 5% CO2. si-RNA targeting HEIH, and corresponding negative control si-NC, miR-194-5p mimics, inhibitor and negative control, WEE1 overexpressing vector pcDNA3.1- WEE1 and negative control pcDNA3.1 were supplied by RiboBio (Guangzhou, China).

Techniques: Expressing, Binding Assay, Luciferase, Activity Assay, Transfection, Negative Control, Control

Journal: OncoTargets and therapy

Article Title: Long Noncoding RNA HEIH Promotes Proliferation, Migration and Invasion of Retinoblastoma Cells Through miR-194-5p/WEE1 Axis

doi: 10.2147/OTT.S268942

Figure Lengend Snippet: HEIH positively regulated WEE1 expression via miR-194-5p. ( A ) Predicted binding site of miR-194-5p in WEE1 3ʹUTR using TargetScan v7.2. ( B and C ) Relative luciferase activity in Y79 ( B ) and SO-Rb50 ( C ) cells co-transfected with wt- WEE1 or mut- WEE1 with miR-194-5p mimics or miRNA negative control. ( D and E ) WEE1 protein levels in Y79 and SO-Rb50 cells transfected with si-HEIH and miR-194-5p inhibitor or miRNA negative control. ( F ) Relative expression level of WEE1 in 35 retinoblastoma tissues and 7 normal retinal tissues. ( G ) Pearson correlation analysis between HEIH and WEE1 mRNA level in retinoblastoma tissues. **P<0.01.

Article Snippet: Human normal retinal epithelial cell line ARPE-19 and retinoblastoma cell lines (Y79 and SO-Rb50) were purchased from ATCC and maintained in RPMI-1640 medium supplemented with 10% FBS at 37°C in 5% CO2. si-RNA targeting HEIH, and corresponding negative control si-NC, miR-194-5p mimics, inhibitor and negative control, WEE1 overexpressing vector pcDNA3.1- WEE1 and negative control pcDNA3.1 were supplied by RiboBio (Guangzhou, China).

Techniques: Expressing, Binding Assay, Luciferase, Activity Assay, Transfection, Negative Control

Journal: OncoTargets and therapy

Article Title: Long Noncoding RNA HEIH Promotes Proliferation, Migration and Invasion of Retinoblastoma Cells Through miR-194-5p/WEE1 Axis

doi: 10.2147/OTT.S268942

Figure Lengend Snippet: Overexpression of WEE1 reversed malignant phenotypes inhibition of retinoblastoma cells induced by HEIH knockdown. ( A ) and ( B ) Western blot was performed to validate WEE1 overexpression. ( C ) Cell viability was detected using the trypan blue exclusion method in Y79 and SO-Rb50 cells co-transfected si-HEIH or siRNA control with pcDNA 3.1- WEE1 or pcDNA 3.1-NC. ( D ) Cell growth capacity was evaluated using the colony formation assay. ( E ) Effects of WEE1 overexpression on cell migration inhibition-induced by HEIH knockdown were determined using wound-healing assays. ( F ) Effects of WEE1 overexpression on cell invasion inhibition-induced by HEIH knockdown were determined using transwell invasion assays. **P<0.01.

Article Snippet: Human normal retinal epithelial cell line ARPE-19 and retinoblastoma cell lines (Y79 and SO-Rb50) were purchased from ATCC and maintained in RPMI-1640 medium supplemented with 10% FBS at 37°C in 5% CO2. si-RNA targeting HEIH, and corresponding negative control si-NC, miR-194-5p mimics, inhibitor and negative control, WEE1 overexpressing vector pcDNA3.1- WEE1 and negative control pcDNA3.1 were supplied by RiboBio (Guangzhou, China).

Techniques: Over Expression, Inhibition, Knockdown, Western Blot, Transfection, Control, Colony Assay, Migration